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BEGIN:VEVENT
DTSTAMP:20260508T114349Z
DTSTART;VALUE=DATE-TIME:20221124T140000
DTEND;VALUE=DATE-TIME:20221124T150000
SUMMARY:SLS/WMS Dev Biol and Stem Cell Seminar Series: Larks of the genom
 e in the soma and in primordial germ cells\, Professor Ferenc Mueller\, 
 Institute of Cancer and Genomic Sciences\, University of Birmingham
TZID:Europe/London
UID:20221124-8a1785d8847a84e201848afae71f4dd0@warwick.ac.uk
CREATED:20221118T134329Z
DESCRIPTION:Abstract Initial development of the embryo is exclusively und
 er the control of maternally deposited proteins and RNAs. In externally 
 developing metazoan embryos cycles of extremely fast and synchronous cel
 l divisions precedes global zygotic genome activation (ZGA). The genome 
 is activated when a threshold nucleo-cytoplasmic ratio is reached\, whic
 h dilutes repressive maternal factors. This event coincides with the mid
 -blastula transition (MBT). However\, the earliest expressed genes\, whi
 ch prepare the rest of the genome for activation escape global genome re
 pression and are activated well before the MBT. How do these genes get t
 ranscribed during the short cell cycles\, which are only 15 minutes and 
 lack G phases\, remains mostly unexplored. We have developed a native tr
 anscription imaging tool\, which detected nascent transcripts accumulati
 ng in a transcription body during the minor wave of genome activation. W
 e described the spatial and temporal dynamics of the first wave of genom
 e activation and its cross-regulation by the cell cycle. We demonstrate 
 that the transcription body forms at an excessively high copy number gen
 e cluster\, composed of the highest promoter density in the genome and w
 hich encodes miR430 microRNAs responsible for clearance of maternal mRNA
 s. While this transcription body represent the main sites of detectable 
 transcription\, we showed that minor wave genes can be independently tra
 nscribed due to their distinct promoter architecture and promoter featur
 es from that of the main wave of ZGA. These findings suggest distinct tr
 anscription mechanisms between the minor and major waves of genome activ
 ation. To further explore mechanisms of zygotic genome activation and th
 e contribution of parental information to this process we also focussed 
 on the formation and specification of primordial germ cells\, which are 
 singled out by selective retention of maternal determinants. 4D imaging 
 and epigenomic analysis of these cells have uncovered the role of cytopl
 asmic maternal determinants in distinct chromatin topology organisation 
 for specific gene regulation of primordial germ cells during early devel
 opment. Selected references 1. Hadzhiev\, Y Wheatley\, L.\, Cooper\, L.\
 , Ansaloni\, F.\, Whalley\, C.\, Chen\, Z.\, Finaurini\, S.\, Gustincich
 \, S.\, Sanges\, R.\, Burgess\, S.\, Beggs\, A. and Müller\, F. (2022) T
 he miR-430 locus with extreme promoter density forms a transcription bod
 y during the minor wave of zygotic genome activation\, in press 2. Baran
 asic D\, Hörtenhuber M\, Balwierz PJ\, Zehnder T\, Mukarram AK\, Nepal C
 \, Várnai C\, Hadzhiev Y\, Jimenez-Gonzalez A\, Li N\, Wragg J\, D'Orazi
 o FM\, Relic D\, Pachkov M\, Díaz N\, Hernández-Rodríguez B\, Chen Z\, …
  et al\,…. Carninci P\, Ohler U\, Lacadie SA\, Burgess SM\, Winata C\, v
 an Eeden F\, Vaquerizas JM\, Gómez-Skarmeta JL\, Onichtchouk D\, Brown B
 J\, Bogdanovic O\, van Nimwegen E\, Westerfield M\, Wardle FC\, Daub CO\
 , Lenhard B and Müller F. (2022) Multiomic atlas with functional stratif
 ication and developmental dynamics of zebrafish cis-regulatory elements.
  Nat Genet. 54(7):1037-1050. 3. D'Orazio FM\, Balwierz PJ\, González AJ\
 , Guo Y\, Hernández-Rodríguez B\, Wheatley\, L\, Jasiulewicz A\, Hadzhie
 v Y\, Vaquerizas JM\, Cairns B\, Lenhard B\, Müller F. (2021) Germ cell 
 differentiation requires Tdrd7-dependent chromatin and transcriptome rep
 rogramming marked by germ plasm relocalization. Dev Cell. 56(5):641-656.
 e5. 4. Hadzhiev\, Y.\, Qureshi\, H.\, Wheatley\, L.\, Cooper\, L. Jasiul
 ewicz\, A.\, Wragg\, J.\, Nguyen\, H.\, Poovathumkadavil\, D.\, Conic\, 
 S.\, Bajan\, S.\, Sik\, A.\, Hutvagner\, G.\, Tora\, L.\, Gambus\, A.\, 
 Fossey J. and Müller F. (2019) A cell cycle-coordinated nuclear compartm
 ent for Polymerase II transcription encompasses the earliest gene expres
 sion before global genome activation Nature Communications 2019 Feb 11\;
 10(1):691. 5. Haberle\, V.\, Li\, N.\, Hadzhiev\, Y.\, Plessy\, C.\, Pre
 viti\, C.\, Nepal\, C.\, Gehrig\, J.\, Dong\, X.\, Akalin\, A.\, Suzuki\
 , A-M.\, van IJcken\, W.\, Armant\, O.\, Ferg\, M.\, Strähle\, U.\, Carn
 inci\, P.\, Müller F.* and Lenhard B. Two independent transcription init
 iation codes overlap on vertebrate core promoters. Nature\, 507(7492):38
 1-5\, *co-corresponding author.
LOCATION:GLT1\, WMS
URL:
ATTACH:
CATEGORIES:BiomedicalSciences,DivisionalSeminars,SLS/WMS Dev Biol and Ste
 m Cell Seminar
LAST-MODIFIED:20221118T134329Z
ORGANIZER;CN=Jas Bains:
END:VEVENT
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