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BEGIN:VEVENT
DTSTAMP:20260507T205637Z
DTSTART;VALUE=DATE-TIME:20230222T131500
DTEND;VALUE=DATE-TIME:20230222T141500
SUMMARY:BMS Seminar: Super-resolution microscopy provides insights into v
 irus spatial organisation\, Dr Nicole Robb\, Division of Biomedical Scie
 nces\, WMS
TZID:Europe/London
UID:20230222-8a17841b83178d8801833786cff65ee3@warwick.ac.uk
CREATED:20230208T144152Z
DESCRIPTION:Abstract: The influenza virus is a major cause of morbidity a
 nd mortality\, and results in a significant health and economic burden w
 orldwide. Despite this\, many aspects of the virus are poorly understood
 . In particular\, filamentous forms of the virus are generally overlooke
 d in virus research\, despite being highly clinically relevant. In addit
 ion\, commonly used methods to study virus structure are often low throu
 gh-put\, or lack the resolution required to resolve virus particle featu
 res. In order to address this\, we have developed a fluorescence super-r
 esolution microscopy and rapid automated analysis pipeline to image many
  thousands of individual influenza virions at a time\, gaining informati
 on on their size\, morphology and protein distribution. Using this pipel
 ine\, we have investigated the size distribution of spherical influenza 
 virions through clustering analysis of super-resolution images\, the len
 gth distribution of influenza filaments by fitting splines to microscopy
  images of filaments\, and the distribution of viral surface proteins an
 d RNA. We observed broad phenotypic variability in filament size\, and F
 ourier transform analysis of super resolution images demonstrated no gen
 eralized common spatial frequency patterning of proteins on the virion s
 urface\, suggesting a model of virus particle assembly where the release
  of progeny filaments from cells occurs in a stochastic way. This work p
 rovides novel insights into virus particle structure and organisation an
 d represents a powerful technique that is easily extendable to the study
  of pleomorphism in other pathogens. Biography: Nicole Robb is an Assist
 ant Professor at the ÌÇÐÄTV Medical School and a visiting lecturer at t
 he University of Oxford. She completed a BSc in Microbiology at Imperial
  College London before doing a DPhil at the University of Oxford\, where
  she specialised in the field of influenza virology. Following a post-do
 c in Physics at the University of Oxford she was awarded a Royal Society
  Dorothy Hodgkin Fellowship to set up her group and moved to the Univers
 ity of ÌÇÐÄTV. Her current research interests include super-resolution 
 microscopy\, rapid pathogen detection and using biophysical techniques t
 o study viral replication. She holds several patents on viral diagnostic
  technology and is co-founder of a spinout company that is working on ra
 pid viral testing. She is also PhD Course Director of the University of 
 ÌÇÐÄTV’s Institute for Global Pandemic Planning (IGPP)\, a new interdis
 ciplinary initiative formed to research solutions to mitigate the effect
 s of pandemics.
LOCATION:GLT1\, Medical School Building
CATEGORIES:BiomedicalSciences,DivisionalSeminars
LAST-MODIFIED:20230208T144152Z
ORGANIZER;CN=Jas Bains:
END:VEVENT
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