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BEGIN:VEVENT
DTSTAMP:20260508T201236Z
DTSTART;VALUE=DATE-TIME:20220705T120000
DTEND;VALUE=DATE-TIME:20220705T130000
SUMMARY:SLS and WMS Biomedical Microbiology Seminar: Molecular Characteri
 zation of colistin and tigecycline resistant classic and hypervirulent K
 lebsiella pneumoniae\, Dr Shanthini Thamaraiselvan\, University of Warwi
 ck
TZID:Europe/London
UID:20220705-8a17841b81a9edc40181b9da694f4a66@warwick.ac.uk
CREATED:20220701T130312Z
DESCRIPTION:Abstract: Klebsiella pneumoniae is the significant opportunis
 tic pathogens from Enterobacteriaceae family which cause both community 
 (non-hospital) and hospital-acquired infections. Our study focuses on th
 e molecular characterization of the colistin and tigecycline-resistant K
 . pneumoniae and its virulence from Tamil Nadu. A total of 315 clinical 
 samples were collected from Trichy\, Madurai\, and Chennai\, from which 
 93 K. pneumoniae were isolated. On subjecting to the antibiotic suscepti
 bility testing\, it was found that 83% were MDR. It was studied that 62%
  were resistant to colistin\, and all the isolates were susceptible to t
 igecycline by Minimum Inhibitory concentration (MIC) broth dilution. Bas
 ed on the Epsilometer test\, the tigecycline heteroresistant (HR) isolat
 es were identified with three subpopulations on the resistant zone. All 
 the K. pneumoniae isolates\, including the subpopulation (n=96)\, were s
 ubjected for detecting hypermucoviscous (HMV) phenotype using string tes
 t and biofilm production using crystal violet staining revealed that 22/
 96 K. pneumoniae isolates were HMV and strong (n=25)\, moderate (n=34)\,
  weak (n=20) and no (n=17) BFPs (BFP). The molecular studies revealed th
 at there were no mcr genes\, class 1 integron (n=23) with devoid of gene
  cassette\, capsular typing K1 (n=5)\, K2 (n=7)\, K5 (n=2)\, Virulence g
 enes namely rmpA (n=5)\, aerobactin (n=12)\, KfuBC (n=12) were identifie
 d using multiplex polymerase chain reaction (PCR). Based on the molecula
 r characterization\, we observed 88 isolates were classical K. pneumonia
 e (cKP)\, and 8 isolates were hypervirulent K. pneumoniae (hvKP). In viv
 o studies were carried out using Galleria mellonella in order to access 
 pathogenicity of cKP and hvKP. The HMV hvKP strains were highly pathogen
 ic among the hvKP strains\, and the non-K1/K2 and K2 strains were pathog
 enic among the cKP strains. The whole-genome shotgun sequencing for the 
 tigecycline heteroresistant K. pneumoniae and the subpopulation that mai
 ntained stability was performed using oxford Nanopore MinIon reveals tha
 t they are extensive drug-resistant (XDR) by analysing the sequence usin
 g Resfinder version 4.0 and are found to be cKP strains using the virule
 nce finder version 2.0. Our data revealed the emergence of CL-R hvKP str
 ains with increased virulence\; the tigecycline heteroresistance was ide
 ntified and characterized at the genomic level for the first time in Tam
 il Nadu\, India.
LOCATION:A151/MBU\, WMS
CATEGORIES:BiomedicalSciences,DivisionalSeminars,Joint WMS/SLS Micro
LAST-MODIFIED:20220701T130312Z
ORGANIZER;CN=Jas Bains:
END:VEVENT
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