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BEGIN:VEVENT
DTSTAMP:20260507T053255Z
DTSTART;VALUE=DATE-TIME:20230925T130000
DTEND;VALUE=DATE-TIME:20230925T140000
SUMMARY:MOB Seminar Series: Shining light on life: from photons to biolog
 ical information\, Dr James Manton\, MRC Laboratory of Molecular Biology
 \, Cambridge
TZID:Europe/London
UID:20230925-8a17841a8a8411e3018a99059caf52d6@warwick.ac.uk
CREATED:20230915T132636Z
DESCRIPTION:Abstract: Fluorescence microscopy has long been a powerful to
 ol for studies in cellular and developmental biology\, but suffers from 
 a number of drawbacks. Chief among these are photobleaching and phototox
 icity\, which limit our ability to see what's going on while maintaining
  the sample in a state compatible with life. In this talk\, I will descr
 ibe our recent work on maximising the ratio of information gained to dam
 age caused\, looking at illumination delivery\, emission handling\, samp
 le preparation and data processing. In particular\, I will describe our 
 implementation of sub-cellular oblique plane microscopy\, which enables 
 gentle 3D imaging over hundreds of timepoints through optimised illumina
 tion. I will then introduce our projection imaging technique\, which can
  increase acquisition rates up to 100-fold\, and our multispectral imagi
 ng system\, capable of imaging eight fluorescence markers with no penalt
 y in spatiotemporal resolution. Examples of how this can be used to stud
 y the dynamics of organelles within living cells and endosomal trafficki
 ng will be shown. This will continue into a discussion of a new hands-fr
 ee deconvolution algorithm\, and conclude with a summary of current work
 -in-progress on improving the stability of fluorescent markers. Biograph
 y: James is a Senior Investigator Scientist and Royal Society University
  Research Fellow at the MRC Laboratory of Molecular Biology\, where he w
 orks to develop new technologies for biological microscopy. Trained as a
  physicist\, his main current interests are in live-cell imaging and flu
 orescence photophysics.
LOCATION:IBRB lecture theatre and via MS Teams
CATEGORIES:BiomedicalSciences,DivisionalSeminars
LAST-MODIFIED:20230915T132636Z
ORGANIZER;CN=Jas Bains:
END:VEVENT
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