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BEGIN:VEVENT
DTSTAMP:20260508T114804Z
DTSTART;VALUE=DATE-TIME:20221129T120000
DTEND;VALUE=DATE-TIME:20221129T130000
SUMMARY:SLS/WMS Microbiology and Infectious Disease seminar series: Click
  and Collect at high resolution to unlock the secrets of cell wall synth
 esis\, Dr Cecile Morlot\, Institut de Biologie Structurale\, France
TZID:Europe/London
UID:20221129-8a17841a849eee2501849f00a67802ec@warwick.ac.uk
CREATED:20221122T110210Z
DESCRIPTION:Abstract: The cell wall is a three-dimensional sugar and pept
 ide network that surrounds the bacterial cell. It confers a cell shape a
 dapted to the ecological niche of the bacterium and protects it against 
 mechanical stress exerted by the environment. Cell wall synthesis and in
 tegrity are thus essential for bacterial proliferation and survival. Des
 pite the importance of these fundamental processes\, which constitute so
 urces of antibiotic targets\, we still poorly understand how the cell wa
 ll is assembled and remodelled in space and time to ensure proper cell d
 ivision\, shape and integrity. This is particularly true for ovoid-shape
 d bacteria such as streptococci and enterococci\, in which two different
  modes of cell wall synthesis\, dedicated to cell division and elongatio
 n\, are confined to an annular region with nanometric dimensions at midc
 ell. Fluorescence microscopy is a method of choice to investigate cell w
 all assembly but it suffers from two major drawbacks. First\, the newly 
 synthesized material must be labelled with a probe that will not perturb
  the physiological process. Second\, the physical properties of light li
 mit the resolution to about 250 nm\, which approximates the dimensions o
 f the cell wall synthesis region. We have met these two challenges by co
 mbining metabolic cell wall labelling (using click chemistry) and super-
 resolution fluorescence microscopy (dSTORM) in the ovoid-shaped human pa
 thogen Streptococcus pneumoniae. Our nanoscale-resolution data (ļ¾ 30 nm)
  unravelled unprecedented spatio-temporal features of cell wall assembly
  and fate along the cell cycle. It provided geometrical and kinetic para
 meters of cell wall synthesis that we further used to simulate the morph
 ogenesis of the ovoid cell in silico. I will present our methodological 
 strategy and the major insights that our experimental and modelling anal
 yses revealed into cell wall synthesis and morphogenesis in ovococci. Bi
 ography: I started studying morphogenesis and division processes in Stre
 ptococcus pneumoniae during my Ph.D. at the Institute for Structural Bio
 logy (2000-2003\, IBS\, Grenoble\, FR). Under the supervision of Thierry
  Vernet\, I investigated the cellular localization of the Penicillin-Bin
 ding Proteins and the structure of the cell wall hydrolase DacA. For my 
 1st post-doc\, I joined the Grenoble EMBL outstation in Stephen Cusackā€™s
  group (2004-2007\, EMBL\, Grenoble\, FR) where I characterized the stru
 cture of a protein complex involved in neuron development in humans. For
  my 2nd post-doc\, I joined the group of David Rudner at Harvard Medical
  School (2007-2010\, HMS\, Boston\, USA) to study the function of two ma
 cromolecular complexes (SpoIIIA-SpoIIQ and SpoIIP-SpoIID) during sporula
 tion in Bacillus subtilis. I was recruited in 2010 as a CNRS researcher 
 and since January 2021\, I took the head of the Pneumococcus group at th
 e IBS (Grenoble\, France). My group employs complementary techniques in 
 microbial genetics\, biochemistry\, structural biology (crystallography\
 , electron microscopy) and cell imaging (including super-resolution fluo
 rescence microscopy) to study the mechanisms of cell morphogenesis durin
 g vegetative growth and sporulation. We address in particular the synthe
 sis of the two main components of the cell wall in the human pathogen S.
  pneumoniae\, the peptidoglycan and the teichoic acids. In parallel\, we
  study the structure and the function of the SpoIIIA-SpoIIQ complex\, a 
 putative new type of secretion system required for spore development in 
 B. subtilis.
LOCATION:A0.30 WMS
CATEGORIES:BiomedicalSciences,DivisionalSeminars,SLS/WMS Microbiology and
  Infectious Disease seminar
LAST-MODIFIED:20221122T110210Z
ORGANIZER;CN=Jas Bains:
END:VEVENT
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