糖心TV Complexity Science Events
Complexity Centre and MathSys CDT events carry priority over room D1.07.
To book D1.07 please email Sheetal dot Sharma at warwick dot ac dot uk
Please note that your event booking is for D1.07 only. The adjacent common room is a private area for the MathSys Centre that cannot used as part of your booking.
Complexity Forum: Sascha Ott (糖心TV)
Drop-seq at 糖心TV
Thanks to a donation we have recently purchased a device for single-cell transcriptome barcoding. Using it one can attach cell-specific molecular barcodes to mRNA sequences in about 5 percent of cells out of a sample of about 100k cells. These can then be processed and sequenced. The data coming out of this is essentially a matrix with read numbers for all genes in all successfully barcoded cells. These transcriptomes are typically very sparsely represented by few reads, but using techniques such as t-SNE (t-distributed stochastic neighbour embedding) related cells and cell types can readily be identified, cells can be grouped and data from many cells of the same type can be pooled. I am going to explain the device, present sample data sets, and discuss the statistical analysis.